Antigenotoxicity of Agaricus blazei mushroom organic and aqueous extracts in chromosomal aberration and cytokinesis block micronucleus assays in CHO-k1 and HTC cell

Agaricus blazei (Ab) has become popularly known for its medicinal properties. ScientiWcally, it has been tested with regard to its capacity to protect genetic material against damage. We examined diVerent organic extracts (methanolic extract—ME, hexanic extract—HE and n-butanolic extract—BE) and an aqueous extract (AE) of Ab, for their capacity to induce DNA damage as well as for their protective eVect. Genetic damage was determined by the chromosomal aberration assay (CA) in CHO-k1 cells for all extracts and the cytokinesis block micronucleus assay (CBMN) in non drug-metabolizing (CHO-k1) and drug-metabolizing (HTC) cell lines for extract BE only. The extracts did not show clastogenicity but showed anticlastogenicity. The greatest percent reduction obtained were with BE (105%) and AE (126%) treatments in CA. BE treatment did not display genotoxicity in CHO-k1, but was genotoxic in HTC. However, BE was shown to be antigenotoxic causing decreased micronucleus frequency in HTC and CHO-k1 cells. These results suggest that all the extracts contained protective substances, but in some cases they could show a genotoxic eVect with regard to metabolism. Therefore, these Wndings warrant caution in the use of this mushroom by the population.

Autores

Bellini MF, Angeli JPF, Matuo R, Terezan AP, Ribeiro LR, Mantovani MS (2006) Antigenotoxicity of Agaricus blazei mushroom organic and aqueous extracts in chromosomal aberration and cytokinesis block micronucleus assays in CHO-k1 and HTC cells. Toxicology in Vitro 20, 355-360.


info@imispain.com
+34 986 917 360
Política de privacidad

© 2019 IMISPAIN

Este sitio web utiliza cookies para que usted tenga la mejor experiencia de usuario. Si continúa navegando está dando su consentimiento para la aceptación de las mencionadas cookies y la aceptación de nuestra política de cookies, pinche el enlace para mayor información.plugin cookies

ACEPTAR
Aviso de cookies